Coding

Part:BBa_K1962007:Design

Designed by: Frank Sargent   Group: iGEM16_Dundee   (2016-10-11)


Colicin E9::Ssp1 Chimera


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1543
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Compliant with RFC[10].


Source

The sequence encoding Ssp1 was amplified from a Serratia marcescens genomic DNA template and cloned into BBa_K1962006, which had previously been designed and synthesised.

References

English G, Trunk K, Rao VA, Srikannathasan V, Hunter WN, Coulthurst SJ. (2012) New secreted toxins and immunity proteins encoded within the Type VI secretion system gene cluster of Serratia marcescens. Mol Microbiol. 86(4):921-36. doi: 10.1111/mmi.12028.